![]() This non-specific binding can be caused by insufficient blocking during staining. Secondary-only controls are used to ensure that there is no non-specific binding of the secondary antibody to the sample. The vehicle-only control confirms that the responses observed following treatment with compounds are not due to the vehicle (the substance that the compound is being dissolved in). The negative control is used to understand the background levels of staining, and the native state without treatment. ![]() Positive controls are used to elicit a known response, and to help determine that the cells/biology are responding appropriately. The vehicle control demonstrates that the vehicle (what your compound is dissolved in) does not elicit the response.įigure 1: Compound controls in high content assays. This allows you to assess whether the changes you are seeing are due to the vehicle (for example, DMSO or ethanol) or the compound of interest.
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